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The concentration of proteins in each observation was distinct using a BCA assay appurtenances (Pierce Chemical Co.). DNA binding assays were conducted using a commercial ELISA trappings (CLONTECH Laboratories, Inc.). SDS-PAGE and Western breakdown.
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IHC staining unfailingly exhibitioned that the NQO1 protein was customarily cytoplasmic (Figure 2 ), while solitary no more than three cases becomeed atomic staining in SCCs (Figure 3 F).
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